NSJ Bioreagents

NDUFA8 Antibody

Product Code:
 
NSJ-RQ8150
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NDUFA8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Flow cytometry testing of fixed and permeabilized human U-87 MG cells with NDUFA8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFA8 antibody.
2 / 3
Flow cytometry testing of fixed and permeabilized human 293T cells with NDUFA8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFA8 antibody.
3 / 3
Western blot testing of 1) human HepG2, 2) human 293T, 3) rat heart and 4) mouse heart tissue lysate with NDUFA8 antibody. Predicted molecular weight ~20 kDa.
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Flow cytometry testing of fixed and permeabilized human U-87 MG cells with NDUFA8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFA8 antibody.
Flow cytometry testing of fixed and permeabilized human 293T cells with NDUFA8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFA8 antibody.
Western blot testing of 1) human HepG2, 2) human 293T, 3) rat heart and 4) mouse heart tissue lysate with NDUFA8 antibody. Predicted molecular weight ~20 kDa.

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CodeSizePrice
NSJ-RQ8150-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 is an enzyme that in humans is encoded by the NDUFA8 gene. The protein encoded by this gene belongs to the complex I 19 kDa subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-K172) was used as the immunogen for the NDUFA8 antibody.
Purity:
Antigen affinity purified
Uniprot #:
P51970