NSJ Bioreagents

PSMC4 Antibody / 26S proteasome regulatory subunit 6B / TBP-7

Product Code:
 
NSJ-RQ8151
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PSMC4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 8
IHC staining of FFPE human lung adenocarcinoma tissue with PSMC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 8
IHC staining of FFPE human rectum adenocarcinoma tissue with PSMC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human glioblastoma tissue with PSMC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human urothelial carcinoma tissue with PSMC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE mouse colon tissue with PSMC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
Immunofluorescent staining of FFPE human U-2 OS cells with PSMC4 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 8
Western blot testing of 1) human HepG2, 2) human U-251, 3) human U-251, 4) human A549, 5) rat liver, 6) rat brain, 7) mouse liver and 8) mouse brain tissue lysate with PSMC4 antibody. Predicted molecular weight: 44-47 kDa (multiple isoforms).
8 / 8
Flow cytometry testing of fixed and permeabilized human Raji cells with PSMC4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSMC4 antibody.
❮❯

IHC staining of FFPE human lung adenocarcinoma tissue with PSMC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectum adenocarcinoma tissue with PSMC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human glioblastoma tissue with PSMC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human urothelial carcinoma tissue with PSMC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse colon tissue with PSMC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with PSMC4 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HepG2, 2) human U-251, 3) human U-251, 4) human A549, 5) rat liver, 6) rat brain, 7) mouse liver and 8) mouse brain tissue lysate with PSMC4 antibody. Predicted molecular weight: 44-47 kDa (multiple isoforms).
Flow cytometry testing of fixed and permeabilized human Raji cells with PSMC4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSMC4 antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ8151-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
26S protease regulatory subunit 6B, also known as 26S proteasome AAA-ATPase subunit Rpt3 and Tat-binding protein 7 (TBP-7), is an enzyme that in humans is encoded by the PSMC4 gene. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-Q412) was used as the immunogen for the PSMC4 antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P43686