NSJ Bioreagents

R°CK1 Antibody / Rho-ass°Ciated protein kinase 1

Product Code:
 
NSJ-RQ8152
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the R°CK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human U-251 cells with ROCK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ROCK1 antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human 293T, 3) rat brain, 4) rat C6, 5) mouse brain and 6) mouse NIH 3T3 cell lysate with ROCK1 antibody. Predicted molecular weight ~158 kDa.
❮❯

Flow cytometry testing of fixed and permeabilized human U-251 cells with ROCK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ROCK1 antibody.
Western blot testing of 1) human HeLa, 2) human 293T, 3) rat brain, 4) rat C6, 5) mouse brain and 6) mouse NIH 3T3 cell lysate with ROCK1 antibody. Predicted molecular weight ~158 kDa.

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CodeSizePrice
NSJ-RQ8152-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R510-D1303) was used as the immunogen for the ROCK1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q13464