NSJ Bioreagents

PSMD7 Antibody / 26S proteasome non-ATPase regulatory subunit 7

Product Code:
 
NSJ-RQ8161
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PSMD7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human MCF7 cells with PSMD7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSMD7 antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human K562, 3) human MCF7, 4) human Caco-2, 5) rat liver, 6) rat RH35, 7) mouse liver and 8) mouse NIH 3T3 cell lysate with PSMD7 antibody. Predicted molecular weight ~37 kDa.
❮❯

Flow cytometry testing of fixed and permeabilized human MCF7 cells with PSMD7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSMD7 antibody.
Western blot testing of 1) human HeLa, 2) human K562, 3) human MCF7, 4) human Caco-2, 5) rat liver, 6) rat RH35, 7) mouse liver and 8) mouse NIH 3T3 cell lysate with PSMD7 antibody. Predicted molecular weight ~37 kDa.

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NSJ-RQ8161-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
26S proteasome non-ATPase regulatory subunit 7, also known as 26S proteasome non-ATPase subunit Rpn8 and Mov34 protein homolog (MOV34, MOV34L), is an enzyme that in humans is encoded by the PSMD7 gene. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-N274) was used as the immunogen for the PSMD7 antibody.
Purity:
Antigen affinity purified
Uniprot #:
P51665