NSJ Bioreagents

RXRA Antibody / Retinoid X Receptor alpha

Product Code:
 
NSJ-RQ8162
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RXR alpha antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human HeLa cells with RXR alpha antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RXR alpha antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human K562, 3) human A431, 4) human HepG2 and 5) rat PC-12 cell lysate with RXR alpha antibody. Predicted molecular weight: ~51 kDa but routinely observed at 54~60 kDa.
❮❯

Flow cytometry testing of fixed and permeabilized human HeLa cells with RXR alpha antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RXR alpha antibody.
Western blot testing of 1) human HeLa, 2) human K562, 3) human A431, 4) human HepG2 and 5) rat PC-12 cell lysate with RXR alpha antibody. Predicted molecular weight: ~51 kDa but routinely observed at 54~60 kDa.

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NSJ-RQ8162-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Retinoid X receptor alpha (RXR-alpha), also known as NR2B1 (nuclear receptor subfamily 2, group B, member 1) is a nuclear receptor that in humans is encoded by the RXRA gene. Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-H133) was used as the immunogen for the RXR alpha antibody.
Purity:
Antigen affinity purified
Uniprot #:
P19793