NSJ Bioreagents

XPA Antibody / DNA repair protein complementing XP-A cells

Product Code:
 
NSJ-RQ8170
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the XPA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human HeLa cells with XPA antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) 293T, 2) HeLa, 3) MCF7 and 4) A549 cell lysate with XPA antibody. Predicted molecular weight ~31 kDa, commonly observed at 31-40 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human 293T cells with XPA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= XPA antibody.
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Immunofluorescent staining of FFPE human HeLa cells with XPA antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) 293T, 2) HeLa, 3) MCF7 and 4) A549 cell lysate with XPA antibody. Predicted molecular weight ~31 kDa, commonly observed at 31-40 kDa.
Flow cytometry testing of fixed and permeabilized human 293T cells with XPA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= XPA antibody.

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CodeSizePrice
NSJ-RQ8170-100ug100 ug£535.00
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This product comes from: United States.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
DNA repair protein complementing XP-A cells is a protein that in humans is encoded by the XPA gene. This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D5-M273) was used as the immunogen for the XPA antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P23025