NSJ Bioreagents

NELFE Antibody / Negative elongation factor E / RD

Product Code:
 
NSJ-RQ8172
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NELFE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
Immunofluorescent staining of FFPE human A549 cells with NELFE antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE human lung squamous cell carcinoma tissue with NELFE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human ovarian cancer tissue with NELFE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) 293T, 2) HeLa and 3) U-2 OS cell lysate with NELFE antibody. Predicted molecular weight ~43 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human 293T cells with NELFE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NELFE antibody.
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Immunofluorescent staining of FFPE human A549 cells with NELFE antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung squamous cell carcinoma tissue with NELFE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with NELFE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) 293T, 2) HeLa and 3) U-2 OS cell lysate with NELFE antibody. Predicted molecular weight ~43 kDa.
Flow cytometry testing of fixed and permeabilized human 293T cells with NELFE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NELFE antibody.

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CodeSizePrice
NSJ-RQ8172-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Negative elongation factor E (NELFE), also called RNA-binding protein RD, is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids N262-D378) was used as the immunogen for the NELFE antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P18615