NSJ Bioreagents

NCOR2 Antibody / Nuclear receptor corepressor 2 / SMRT

Product Code:
 
NSJ-RQ8182
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NCOR2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with NCOR2 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of human 1) U-2 OS, 2) U-251, 3) HeLa and 4) PC-3 cell lysate with NCOR2 antibody. Predicted molecular weight: 267-274 kDa, ~81 kDa (multiple isoforms).
3 / 4
Flow cytometry testing of fixed and permeabilized human RT4 cells with NCOR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NCOR2 antibody.
4 / 4
Flow cytometry testing of fixed and permeabilized human HeLa cells with NCOR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NCOR2 antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with NCOR2 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) U-2 OS, 2) U-251, 3) HeLa and 4) PC-3 cell lysate with NCOR2 antibody. Predicted molecular weight: 267-274 kDa, ~81 kDa (multiple isoforms).
Flow cytometry testing of fixed and permeabilized human RT4 cells with NCOR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NCOR2 antibody.
Flow cytometry testing of fixed and permeabilized human HeLa cells with NCOR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NCOR2 antibody.

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CodeSizePrice
NSJ-RQ8182-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
This gene encodes a nuclear receptor co-repressor that mediates transcriptional silencing of certain target genes. The encoded protein is a member of a family of thyroid hormone- and retinoic acid receptor-associated co-repressors. This protein acts as part of a multisubunit complex which includes histone deacetylases to modify chromatin structure that prevents basal transcriptional activity of target genes. Aberrant expression of this gene is associated with certain cancers. Alternate splicing results in multiple transcript variants encoding different isoforms.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q6-H2148) was used as the immunogen for the NCOR2 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q9Y618