NSJ Bioreagents

N4BP1 Antibody / NEDD4-binding protein 1 / KIAA0615

Product Code:
 
NSJ-RQ8184
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the N4BP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human HeLa cells with N4BP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HepG2, 2) RT4 and 3) Jurkat cell lysate with N4BP1 antibody. Predicted molecular weight ~100 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human JK cells with N4BP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= N4BP1 antibody.
❮❯

Immunofluorescent staining of FFPE human HeLa cells with N4BP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HepG2, 2) RT4 and 3) Jurkat cell lysate with N4BP1 antibody. Predicted molecular weight ~100 kDa.
Flow cytometry testing of fixed and permeabilized human JK cells with N4BP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= N4BP1 antibody.

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CodeSizePrice
NSJ-RQ8184-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73?, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73? for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73? and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-K885) was used as the immunogen for the N4BP1 antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O75113