NSJ Bioreagents

PSMD6 Antibody / 26S proteasome non-ATPase regulatory subunit 6

Product Code:
 
NSJ-RQ8185
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PSMD6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with PAPSS2 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of human 1) HeLa, 2) RT4, 3) U-251 and 4) Jurkat cell lysate with PSMD6 antibody. Predicted molecular weight: 41-52 kDa (multiple isoforms).
3 / 4
Flow cytometry testing of fixed and permeabilized human MCF7 cells with PSMD6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSMD6 antibody.
4 / 4
Flow cytometry testing of fixed and permeabilized human JK cells with PSMD6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSMD6 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with PAPSS2 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) RT4, 3) U-251 and 4) Jurkat cell lysate with PSMD6 antibody. Predicted molecular weight: 41-52 kDa (multiple isoforms).
Flow cytometry testing of fixed and permeabilized human MCF7 cells with PSMD6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSMD6 antibody.
Flow cytometry testing of fixed and permeabilized human JK cells with PSMD6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSMD6 antibody.

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CodeSizePrice
NSJ-RQ8185-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
26S proteasome non-ATPase regulatory subunit 6 is an enzyme that in humans is encoded by the PSMD6 gene. This gene encodes a member of the protease subunit S10 family. The encoded protein is a subunit of the 26S proteasome which colocalizes with DNA damage foci and is involved in the ATP-dependent degradation of ubiquinated proteins. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E30-Q381) was used as the immunogen for the PSMD6 antibody.
Localisation:
Nuclear, cytoplasmic, extracellular
Purity:
Antigen affinity purified
Uniprot #:
Q15008