NSJ Bioreagents

NEK6 Antibody / NimA-related protein kinase 6

Product Code:
 
NSJ-RQ8190
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NEK6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with NEK6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HepG2, 2) HeLa, 3) 293T and 4) HT-1080 cell lysate with NEK6 antibody. Predicted molecular weight: 35-40 kDa (multiple isoforms), commonly observed at 35-45 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human HepG2 cells with NEK6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NEK6 antibody.
❮❯

Immunofluorescent staining of FFPE human A549 cells with NEK6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HepG2, 2) HeLa, 3) 293T and 4) HT-1080 cell lysate with NEK6 antibody. Predicted molecular weight: 35-40 kDa (multiple isoforms), commonly observed at 35-45 kDa.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with NEK6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NEK6 antibody.

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CodeSizePrice
NSJ-RQ8190-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Serine/threonine-protein kinase Nek6 is an enzyme that in humans is encoded by the NEK6 gene. The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids N16-T313) was used as the immunogen for the NEK6 antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9HC98