NSJ Bioreagents

CEP164 Antibody / Centrosomal protein of 164 kDa

Product Code:
 
NSJ-RQ8196
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the CEP164 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human HeLa cells with CEP164 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of human 1) K562 and 2) SiHa cell lysate with CEP164 antibody. Predicted molecular weight ~164 kDa.
3 / 4
Flow cytometry testing of fixed and permeabilized human RT4 cells with CEP164 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CEP164 antibody.
4 / 4
Flow cytometry testing of fixed and permeabilized human K562 cells with CEP164 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CEP164 antibody.
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Immunofluorescent staining of FFPE human HeLa cells with CEP164 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) K562 and 2) SiHa cell lysate with CEP164 antibody. Predicted molecular weight ~164 kDa.
Flow cytometry testing of fixed and permeabilized human RT4 cells with CEP164 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CEP164 antibody.
Flow cytometry testing of fixed and permeabilized human K562 cells with CEP164 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CEP164 antibody.

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CodeSizePrice
NSJ-RQ8196-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Centrosomal protein of 164 kDa, also known as CEP164, is a protein that in humans is encoded by the CEP164 gene. This gene encodes a centrosomal protein involved in microtubule organization, DNA damage response, and chromosome segregation. The encoded protein is required for assembly of primary cilia and localizes to mature centrioles. Defects in this gene are a cause of nephronophthisis-related ciliopathies. Two transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D10-R903) was used as the immunogen for the CEP164 antibody.
Localisation:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q9UPV0