NSJ Bioreagents

RUFY3 Antibody / RUN and FYVE domain-containing protein 3 / RIPx

Product Code:
 
NSJ-RQ8197
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RUFY3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with RUFY3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RUFY3 antibody.
2 / 2
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with RUFY3 antibody. Predicted molecular weight: 53-70 kDa (multiple isoforms).
❮❯

Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with RUFY3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RUFY3 antibody.
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with RUFY3 antibody. Predicted molecular weight: 53-70 kDa (multiple isoforms).

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CodeSizePrice
NSJ-RQ8197-100ug100 ug£535.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
The RUFY3 gene, also called RUN and FYVE domain-containing protein 3 and Rap2-interacting protein x (RIPx), encodes a RPIP8, UNC-14, and NESCA domain-containing protein that is required for maintenance of neuronal polarity. In addition, it has been implicated in mediation of gastric cancer cell migration and invasion via interaction with P21-activated kinase-1, which promotes its expression. The encoded protein localizes to F-actin-enriched invadopodia to induce formation of protrusions, thereby facilitating cell migration. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-H469) was used as the immunogen for the RUFY3 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q7L099