NSJ Bioreagents

NCAPH Antibody / Condensin complex subunit 2

Product Code:
 
NSJ-RQ8201
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NCAPH antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with NCAPH antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HeLa, 2) 293T and 3) K562 cell lysate with NCAPH antibody. Predicted molecular weight: ~83 kDa, routinely observed at 90-97 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human 293T cells with NCAPH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NCAPH antibody.
❮❯

Immunofluorescent staining of FFPE human A549 cells with NCAPH antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) 293T and 3) K562 cell lysate with NCAPH antibody. Predicted molecular weight: ~83 kDa, routinely observed at 90-97 kDa.
Flow cytometry testing of fixed and permeabilized human 293T cells with NCAPH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NCAPH antibody.

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CodeSizePrice
NSJ-RQ8201-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Condensin complex subunit 2 also known as chromosome-associated protein H (CAP-H) or non-SMC condensin I complex subunit H (NCAPH) is a protein that in humans is encoded by the NCAPH gene. This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R91-K721) was used as the immunogen for the NCAPH antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q15003