NSJ Bioreagents

PPWD1 Antibody / Peptidylprolyl isomerase domain and WD repeat-containing protein 1

Product Code:
 
NSJ-RQ8202
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PPWD1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE mouse brain tissue with PPWD1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE rat brain tissue with PPWD1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Immunofluorescent staining of FFPE human U-2 OS cells with PPWD1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 5
Western blot testing of 1) human 293T, 2) human HeLa, 3) human Jurkat, 4) rat brain, 5) rat kidney, 6) mouse brain, 7) mouse kidney and 8) mouse SP2/0 cell lysate with PPWD1 antibody. Predicted molecular weight ~74 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human 293T cells with PPWD1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPWD1 antibody.
❮❯

IHC staining of FFPE mouse brain tissue with PPWD1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with PPWD1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with PPWD1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human HeLa, 3) human Jurkat, 4) rat brain, 5) rat kidney, 6) mouse brain, 7) mouse kidney and 8) mouse SP2/0 cell lysate with PPWD1 antibody. Predicted molecular weight ~74 kDa.
Flow cytometry testing of fixed and permeabilized human 293T cells with PPWD1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPWD1 antibody.

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CodeSizePrice
NSJ-RQ8202-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D188-N529) was used as the immunogen for the PPWD1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q96BP3