NSJ Bioreagents

Fox2 Antibody / RBM9 / RBFOX2

Product Code:
 
NSJ-RQ8204
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RBFOX2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 9
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with RBFOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 9
IHC staining of FFPE human liver cancer tissue with RBFOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 9
IHC staining of FFPE human esophageal squamous carcinoma tissue with RBFOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human cortical adenoma tissue with RBFOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human lung cancer tissue with RBFOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE mouse kidney tissue with RBFOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 9
Immunofluorescent staining of FFPE human lung cancer tissue with RBFOX2 antibody (red). HIER: steam section in pH8 EDTA buffer for 20 min.
8 / 9
Western blot testing of 1) human U-2 OS, 2) Caco-2, 3) A431, 4) human U-87 MG, 5) rat C6 and 6) mouse NIH 3T3 cell lysate with RBFOX2 antibody. Predicted molecular weight ~41 kDa.
9 / 9
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with RBFOX2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RBFOX2 antibody.
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IHC staining of FFPE human ovarian serous adenocarcinoma tissue with RBFOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with RBFOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with RBFOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cortical adenoma tissue with RBFOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with RBFOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney tissue with RBFOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human lung cancer tissue with RBFOX2 antibody (red). HIER: steam section in pH8 EDTA buffer for 20 min.
Western blot testing of 1) human U-2 OS, 2) Caco-2, 3) A431, 4) human U-87 MG, 5) rat C6 and 6) mouse NIH 3T3 cell lysate with RBFOX2 antibody. Predicted molecular weight ~41 kDa.
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with RBFOX2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RBFOX2 antibody.

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CodeSizePrice
NSJ-RQ8204-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
RNA binding motif protein 9 (RBM9), also known as Rbfox2, is a protein which in humans is encoded by the RBM9 gene. It is mapped to 22q12.3. This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-Y256) was used as the immunogen for the RBFOX2 antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O43251