NSJ Bioreagents

PPIL2 Antibody / Cyp60

Product Code:
 
NSJ-RQ8205
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Cyp60 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with Cyp60 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) 293T and 2) K562 cell lysate with Cyp60 antibody. Predicted molecular weight ~59 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human 293T cells with Cyp60 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cyp60 antibody.
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Immunofluorescent staining of FFPE human A549 cells with Cyp60 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) 293T and 2) K562 cell lysate with Cyp60 antibody. Predicted molecular weight ~59 kDa.
Flow cytometry testing of fixed and permeabilized human 293T cells with Cyp60 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cyp60 antibody.

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CodeSizePrice
NSJ-RQ8205-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Peptidyl-prolyl cis-trans isomerase-like 2, also called Cyclophilin-60 (Cyp60), is an enzyme that in humans is encoded by the PPIL2 gene. This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. Alternatively spliced transcript variants have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D40-Q274) was used as the immunogen for the Cyp60 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q13356