NSJ Bioreagents

SEMA4A Antibody / Semaphorin 4A / Sema B

Product Code:
 
NSJ-RQ8233
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Semaphorin 4A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human JK cells with Semaphorin 4A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Semaphorin 4A antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human RT4, 4) rat thymus, 5) rat PC-12, 6) mouse thymus and 7) mouse ANA-1 cell lysate with Semaphorin 4A antibody. Predicted molecular weight ~84 kDa but may be observed at higher molecular weights due to glycosylation.
❮❯

Flow cytometry testing of fixed and permeabilized human JK cells with Semaphorin 4A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Semaphorin 4A antibody.
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human RT4, 4) rat thymus, 5) rat PC-12, 6) mouse thymus and 7) mouse ANA-1 cell lysate with Semaphorin 4A antibody. Predicted molecular weight ~84 kDa but may be observed at higher molecular weights due to glycosylation.

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CodeSizePrice
NSJ-RQ8233-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Semaphorin-4A is a protein that in humans is encoded by the SEMA4A gene. This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R81-N754) was used as the immunogen for the Semaphorin 4A antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q9H3S1