NSJ Bioreagents

IKBZ Antibody / IKB zeta / NFKBIZ

Product Code:
 
NSJ-RQ8234
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the IKB zeta antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human SiHa cells with IKB zeta antibody (green) and Beta Tubuliln mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) RT4 and 2) Caco-2 cell lysate with IKB zeta antibody. Predicted molecular weight: 64-78 kDa (multiple isoforms), commonly observed at 75-85 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human HEL cells with IKB zeta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IKB zeta antibody.
❮❯

Immunofluorescent staining of FFPE human SiHa cells with IKB zeta antibody (green) and Beta Tubuliln mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) RT4 and 2) Caco-2 cell lysate with IKB zeta antibody. Predicted molecular weight: 64-78 kDa (multiple isoforms), commonly observed at 75-85 kDa.
Flow cytometry testing of fixed and permeabilized human HEL cells with IKB zeta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IKB zeta antibody.

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CodeSizePrice
NSJ-RQ8234-100ug100 ug£535.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
NF-kappa-B inhibitor zeta (IKBz) is a protein that in humans is encoded by the NFKBIZ gene. This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q247-H577) was used as the immunogen for the IKB zeta antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q9BYH8