NSJ Bioreagents

PYROXD1 Antibody / Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1

Product Code:
 
NSJ-RQ8237
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PYROXD1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with PYROXD1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human ThP-1, 2) human SH-SY5Y, 3) rat brain, 4) mouse brain and 5) mouse NIH 3T3 cell lysate with PYROXD1 antibody. Predicted molecular weight ~56 kDa, ~48 kDa (two isoforms).
3 / 3
Flow cytometry testing of fixed and permeabilized human 293T cells with PYROXD1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PYROXD1 antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with PYROXD1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human ThP-1, 2) human SH-SY5Y, 3) rat brain, 4) mouse brain and 5) mouse NIH 3T3 cell lysate with PYROXD1 antibody. Predicted molecular weight ~56 kDa, ~48 kDa (two isoforms).
Flow cytometry testing of fixed and permeabilized human 293T cells with PYROXD1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PYROXD1 antibody.

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NSJ-RQ8237-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Pyridine nucleotide-disulphide oxidoreductase domain 1 is a protein that in humans is encoded by the PYROXD1 gene. It is mapped to 12p12.1. This gene encodes a nuclear-cytoplasmic pyridine nucleotide-disulphide reductase (PNDR). PNDRs are flavoproteins that catalyze the pyridine nucleotide-dependent reduction of thiol residues in other proteins. The encoded protein belongs to the class I pyridine nucleotide-disulphide oxidoreductase family but lacks the C-terminal dimerization domain found in other family members and instead has a C-terminal nitrile reductase domain. It localizes to the nucleus and to striated sarcomeric compartments. Naturally occurring mutations in this gene cause early-onset myopathy with internalized nuclei and myofibrillar disorganization. A pseudogene of this gene has been defined on chromosome 11.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids A26-N453) was used as the immunogen for the PYROXD1 antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q8WU10