NSJ Bioreagents

PPP1R15B Antibody / Protein phosphatase 1 regulatory subunit 15B

Product Code:
 
NSJ-RQ8244
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Protein phosphatase 1 regulatory subunit 15B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human 293T cells with Protein phosphatase 1 regulatory subunit 15B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Protein phosphatase 1 regulatory subunit 15B antibody.
2 / 2
Western blot testing of human 1) 293T, 2) MCF7 and 3) HeLa cell lysate with Protein phosphatase 1 regulatory subunit 15B antibody. Predicted molecular weight ~79 kDa but may be observed at up to ~100 kDa, possibly due to phosphorylation.
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Flow cytometry testing of fixed and permeabilized human 293T cells with Protein phosphatase 1 regulatory subunit 15B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Protein phosphatase 1 regulatory subunit 15B antibody.
Western blot testing of human 1) 293T, 2) MCF7 and 3) HeLa cell lysate with Protein phosphatase 1 regulatory subunit 15B antibody. Predicted molecular weight ~79 kDa but may be observed at up to ~100 kDa, possibly due to phosphorylation.

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CodeSizePrice
NSJ-RQ8244-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
PPP1R15B (Protein phosphatase 1, regulatory subunit 15b), also called CREP, promotes dephosphorylation of the transcription initiation factor EIF2-alpha through recruitment of protein phosphatase-1 (PP1) catalytic subunits. The PPP1R15B gene is mapped to chromosome 1q32.1 based on an alignment of the PPP1R15B sequence by Hartz(2010). Harding et al.(2009) obtained Ppp1r15b -/- mice at a mendelian ratio. However, Ppp1r15b -/- newborns were half the size of their wildtype littermates, were notably pale, and failed to nurse, and none survived the first day of postnatal life. Ppp1r15b -/- embryos that were also homozygous for an Eif2-alpha mutation that prevented Eif2-alpha phosphorylation were normalized, including elevated birth size and restored red blood cell count, compared with Ppp1r15b -/- embryos with wildtype Eif2-alpha.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q211-D657) was used as the immunogen for the Protein phosphatase 1 regulatory subunit 15B antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q5SWA1