NSJ Bioreagents

PRRC1 Antibody / Proline-rich and coiled-coil-containing protein 1

Product Code:
 
NSJ-RQ8248
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PRRC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human HeLa cells with PRRC1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HeLa, 2) MCF7, 3) HepG2 and 4) Caco-2 cell lysate with PRRC1 antibody. Predicted molecular weight ~47 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human U-251 cells with PRRC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRRC1 antibody.
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Immunofluorescent staining of FFPE human HeLa cells with PRRC1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) MCF7, 3) HepG2 and 4) Caco-2 cell lysate with PRRC1 antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of fixed and permeabilized human U-251 cells with PRRC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRRC1 antibody.

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NSJ-RQ8248-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
PRRC1 (Proline-Rich Coiled-Coil 1) is a Protein Coding gene. The PRRC1 gene, located on 5q23.2, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. The PRCC1 gene has two distinct regions: a proline-rich region on the N-terminus, and the DUF84 region on the C-terminus. PRCC1 is the commonly identified protein name of CAD38605. It encodes for 445 amino acids for a predicted total of 6 exons. The predicted molecular weight is 46.7 kDa, and the isoelectric point is 5.46. Orthologs have been determined in most eukaryotes, the most highly conserved being found in most mammalian species. PRRC1 is widely expressed in the thyroid, endometrium, and other tissues. Diseases associated with PRRC1 include Dystonia 23.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Y139-D411) was used as the immunogen for the PRRC1 antibody.
Localisation:
Cytoplasm
Purity:
Antigen affinity purified
Uniprot #:
Q96M27