NSJ Bioreagents

SCFD2 Antibody / Sec1 family domain-containing protein 2

Product Code:
 
NSJ-RQ8250
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SCFD2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human HeLa cells with SCFD2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human U-251, 2) human K562, 3) human PC-3 and 4) mouse kidney tissue lysate with SCFD2 antibody. Predicted molecular weight: 70-75 kDa (two isoforms).
3 / 3
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with SCFD2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SCFD2 antibody.
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Immunofluorescent staining of FFPE human HeLa cells with SCFD2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human U-251, 2) human K562, 3) human PC-3 and 4) mouse kidney tissue lysate with SCFD2 antibody. Predicted molecular weight: 70-75 kDa (two isoforms).
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with SCFD2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SCFD2 antibody.

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CodeSizePrice
NSJ-RQ8250-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
SCFD2 (sec1 family domain containing 2), also known as STXBP1L1 (syntaxinbinding protein 1-like 1), is a 684 amino acid protein suggested to play a role in protein transport. Existing as two alternatively spliced isoforms, SCFD2 is a member of the STXBP/unc-18/SEC1 family and is encoded by a gene that maps to human chromosome 4q12. Chromosome 4 represents approximately 6% of the human genome and contains nearly 900 genes. Notably, the Huntingtin gene, which is found to encode an expanded glutamine tract in cases of Huntington�s disease, is on chromosome 4. FGFR-3 is also encoded on chromosome 4 and has been associated with thanatophoric dwarfism, achondroplasia, Muenke syndrome and bladder cancer. Chromosome 4 is also tied to Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K20-H585) was used as the immunogen for the SCFD2 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q8WU76