NSJ Bioreagents

SERPINB5 Antibody / MASPIN

Product Code:
 
NSJ-V9413SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SERPINB5/4972
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the SERPINB5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC staining of FFPE human skin with SERPINB5 antibody (clone SERPINB5/4972). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human skin tissue with SERPINB5 antibody (clone SERPINB5/4972). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
Western blot testing of human PC3 cell lysate using SERPINB5 antibody (clone SERPINB5/4972). Predicted molecular weight: 42~45 kDa.
4 / 5
Western blot testing of human SW732 cell lysate using SERPINB5 antibody (clone SERPINB5/4972). Predicted molecular weight: 42~45 kDa.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SERPINB5 antibody (clone SERPINB5/4972). These results demonstrate the foremost specificity of the SERPINB5/4972 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

IHC staining of FFPE human skin with SERPINB5 antibody (clone SERPINB5/4972). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin tissue with SERPINB5 antibody (clone SERPINB5/4972). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human PC3 cell lysate using SERPINB5 antibody (clone SERPINB5/4972). Predicted molecular weight: 42~45 kDa.
Western blot testing of human SW732 cell lysate using SERPINB5 antibody (clone SERPINB5/4972). Predicted molecular weight: 42~45 kDa.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SERPINB5 antibody (clone SERPINB5/4972). These results demonstrate the foremost specificity of the SERPINB5/4972 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9413SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Maspin/SERPINB5 is structurally a serine protease inhibitor (serpin) that was initially isolated from normal human mammary epithelial cells. Serpins are a family of proteins that inhibit Chymotrypsin-like serine proteinases. Serpins control activated proteinases and several are involved in the regulation of cell death. Maspin is found in the extracellular matrix and at the plasma membrane. Maspin has been shown to act at the cell surface to block cell motility and inhibit invasion of breast and prostate cancer cells. Maspin is present in normal mammary epithelial cells but is absent in many tumor cell lines, yet no major structural alterations of the Maspin gene have been identified in tumor cells. Similarly, Maspin is expressed in normal prostate cells and downregulated or absent in prostate tumor cells.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids1-200 was used as the immunogen for the SERPINB5 antibody.
Localisation:
Secreted, Extracellular space
Purity:
Protein A/G affinity
Uniprot #:
P36952