NSJ Bioreagents

Recombinant Actin Antibody / ACTA2 / Smooth Muscle

Product Code:
 
NSJ-V9420SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ACTA2/1614R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC staining of FFPE human skeletal muscle with recombinant Actin antibody (clone ACTA2/1614R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human uterus tissue with recombinant Actin (clone ACTA2/1614R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Actin antibody (clone ACTA2/1614R) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human skeletal muscle with recombinant Actin antibody (clone ACTA2/1614R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human uterus tissue with recombinant Actin (clone ACTA2/1614R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Actin antibody (clone ACTA2/1614R) as confirmation of integrity and purity.

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NSJ-V9420SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human ACTA2 protein was used as the immunogen for the recombinant Actin antibody.
Localisation:
Cytoplasmic
Purity:
Protein A/G affinity
Uniprot #:
P62736