NSJ Bioreagents

Recombinant CAD Antibody / CALD1 / Caldesmon

Product Code:
 
NSJ-V9428SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CALD1/7024R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the recombinant CAD antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot testing of human ovary tissue lysate using recombinant CAD antibody (clone CALD1/7024R). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).
2 / 3
IHC staining of FFPE human colon tissue with recombinant CAD antibody (clone CALD1/7024R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant CAD antibody (clone CALD1/7024R) as confirmation of integrity and purity.

Western blot testing of human ovary tissue lysate using recombinant CAD antibody (clone CALD1/7024R). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).
IHC staining of FFPE human colon tissue with recombinant CAD antibody (clone CALD1/7024R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CAD antibody (clone CALD1/7024R) as confirmation of integrity and purity.

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NSJ-V9428SAF-100UG100 ug£534.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human full-length Caldesmon protein was used as the immunogen for the recombinant CAD antibody.
Localisation:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q05682