NSJ Bioreagents

Recombinant CD3 epsilon Antibody

Product Code:
 
NSJ-V9434SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
C3e/4653R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the recombinant CD3 epsilon antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot testing of human Jurkat cell lysate using recombinant CD3 epsilon antibody (clone C3e/4653R). Predicted molecular weight ~23 kDa.
2 / 3
IHC staining of FFPE human tonsil tissue with recombinant CD3e antibody (clone CD3e/4653R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
Negative control: IHC staining of FFPE human brain tissue using recombinant CD3 epsilon antibody (clone C3e/4653R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
❮❯

Western blot testing of human Jurkat cell lysate using recombinant CD3 epsilon antibody (clone C3e/4653R). Predicted molecular weight ~23 kDa.
IHC staining of FFPE human tonsil tissue with recombinant CD3e antibody (clone CD3e/4653R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using recombinant CD3 epsilon antibody (clone C3e/4653R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-V9434SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
Description:
Cluster of differentiation 3 (CD3) is a multimeric protein complex, known historically as the T3 complex, and is composed of four distinct polypeptide chains: epsilon, gamma, delta and zeta. The CD3 complex serves as a T cell co-receptor that associates noncovalently with the T cell receptor (TCR). CD3 antigen appears at all stages of T cell development which makes it an ideal T cell marker for both the detection of normal T cells and T cell neoplasms (lymphomas and leukemias).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 100-200 was used as the immunogen for the recombinant CD3 epsilon antibody.
Localisation:
Cell Surface, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P07766