NSJ Bioreagents

Recombinant CD30 Antibody

Product Code:
 
NSJ-V9437SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
rKi-1/6913
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant CD30 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 2
IHC staining of FFPE human Hodgkins lymphoma tissue with recombinant CD30 antibody (clone rKi-1/6913) at 2ug/ml in PBS for 30min RT. Staining of Reed-Sternberg cells is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 2
SDS-PAGE analysis of purified, BSA-free recombinant CD30 antibody (clone rKi-1/6913) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human Hodgkins lymphoma tissue with recombinant CD30 antibody (clone rKi-1/6913) at 2ug/ml in PBS for 30min RT. Staining of Reed-Sternberg cells is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD30 antibody (clone rKi-1/6913) as confirmation of integrity and purity.

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NSJ-V9437SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. Its epitope is located somewhere between aa 580-595 (cytoplasmic). CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkin s disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This MAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 495-595 was used as the immunogen for the recombinant CD30 antibody.
Localisation:
Cell Surface
Purity:
Protein A affinity
Uniprot #:
P28908