NSJ Bioreagents

Recombinant CD59 Antibody

Product Code:
 
NSJ-V9441
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MACIF/7021R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant CD59 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC staining of FFPE human spleen tissue with recombinant CD59 antibody (clone MACIF/7021R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human tonsil tissue with recombinant CD59 antibody (clone MACIF/7021R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human kidney tissue with recombinant CD59 antibody (clone MACIF/7021R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE human cerebellum tissue with recombinant CD59 antibody (clone MACIF/7021R) at 2ug/ml. Cytoplasmic and membranous staining observed. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 5
SDS-PAGE analysis of purified, BSA-free recombinant CD59 antibody (clone MACIF/7021R) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human spleen tissue with recombinant CD59 antibody (clone MACIF/7021R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant CD59 antibody (clone MACIF/7021R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney tissue with recombinant CD59 antibody (clone MACIF/7021R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cerebellum tissue with recombinant CD59 antibody (clone MACIF/7021R) at 2ug/ml. Cytoplasmic and membranous staining observed. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD59 antibody (clone MACIF/7021R) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9441-20UG20 ug£264.00
Quantity:
NSJ-V9441-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
CD59 regulates complement-mediated cell lysis and it is involved in lymphocyte signal transduction.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant human full-length CD59 protein was used as the immunogen for the recombinant CD59 antibody.
Localisation:
Cell Surface
Purity:
Protein A affinity
Uniprot #:
P13987