NSJ Bioreagents

Recombinant Cdc20 Antibody

Product Code:
 
NSJ-V9446SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CDC20/7026R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant Cdc20 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC staining of FFPE human kidney tissue with recombinant Cdc20 antibody CDC20/7026R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human kidney carcinoma tissue with recombinant Cdc20 antibody (clone CDC20/7026R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Negative control: IHC staining of FFPE human brain tissue with recombinant Cdc20 antibody (clone CDC20/7026R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free recombinant Cdc20 antibody (clone CDC20/7026R) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human kidney tissue with recombinant Cdc20 antibody CDC20/7026R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney carcinoma tissue with recombinant Cdc20 antibody (clone CDC20/7026R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant Cdc20 antibody (clone CDC20/7026R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Cdc20 antibody (clone CDC20/7026R) as confirmation of integrity and purity.

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NSJ-V9446SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human Cdc20 protein was used as the immunogen for the recombinant Cdc20 antibody.
Localisation:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q12834