NSJ Bioreagents

Recombinant DNA topoisomerase I Antibody / TOP1MT

Product Code:
 
NSJ-V9451
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
rTOP1MT/488
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant DNA topoisomerase I antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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IHC staining of FFPE human liver tissue with recombinant DNA topoisomerase I antibody (clone rTOP1MT/488). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 2
SDS-PAGE analysis of purified, BSA-free recombinant DNA topoisomerase I antibody (clone rTOP1MT/488) as confirmation of integrity and purity.
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IHC staining of FFPE human liver tissue with recombinant DNA topoisomerase I antibody (clone rTOP1MT/488). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant DNA topoisomerase I antibody (clone rTOP1MT/488) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9451-20UG20 ug£264.00
Quantity:
NSJ-V9451-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human TOP1MT protein was used as the immunogen for the recombinant DNA topoisomerase I antibody.
Localisation:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q969P6