NSJ Bioreagents

NME1 Antibody / NM23

Product Code:
 
NSJ-V9453
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
NME1/2737
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the NME1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC staining of FFPE human cervix tissue with NME1 antibody (clone NME1/2737). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human breast carcinoma tissue with NME1 antibody (clone NME1/2737). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
Western blot analysis of human 1) A549, 2) PC3 and 3) K562 cell lysates using NME1 antibody (clone NME1/2737). Predicted molecular weight ~17 kDa.
4 / 5
SDS-PAGE analysis of purified, BSA-free NME1 antibody (clone NME1/2737) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using NME1 antibody (clone NME1/2737). These results demonstrate the foremost specificity of the NME1/2737 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

IHC staining of FFPE human cervix tissue with NME1 antibody (clone NME1/2737). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma tissue with NME1 antibody (clone NME1/2737). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot analysis of human 1) A549, 2) PC3 and 3) K562 cell lysates using NME1 antibody (clone NME1/2737). Predicted molecular weight ~17 kDa.
SDS-PAGE analysis of purified, BSA-free NME1 antibody (clone NME1/2737) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using NME1 antibody (clone NME1/2737). These results demonstrate the foremost specificity of the NME1/2737 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9453-20UG20 ug£264.00
Quantity:
NSJ-V9453-100UG100 ug£534.00
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human NME1 protein was used as the immunogen for the NME1 antibody.
Localisation:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P15531