NSJ Bioreagents

CD48 Antibody (Pan Leuk°Cyte Marker)

Product Code:
 
NSJ-V9471
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2c, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CD48/4783
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the CD48 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Western blot testing of human Raji cell lysate using CD48 antibody (clone CD48/4783). Predicted molecular weight: 28-50 kDa depending on glycosylation level.
2 / 5
IHC staining of FFPE human tonsil tissue with CD48 antibody (clone CD48/4783) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human lymph node tissue with CD48 antibody (clone CD48/4783) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
SDS-PAGE analysis of purified, BSA-free CD48 antibody (clone CD48/4783) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD48 antibody (clone CD48/4783). These results demonstrate the foremost specificity of the CD48/4783 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Western blot testing of human Raji cell lysate using CD48 antibody (clone CD48/4783). Predicted molecular weight: 28-50 kDa depending on glycosylation level.
IHC staining of FFPE human tonsil tissue with CD48 antibody (clone CD48/4783) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node tissue with CD48 antibody (clone CD48/4783) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD48 antibody (clone CD48/4783) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD48 antibody (clone CD48/4783). These results demonstrate the foremost specificity of the CD48/4783 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9471-20UG20 ug£264.00
Quantity:
NSJ-V9471-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 59-178 was used as the immunogen for the CD48 antibody.
Localisation:
Cell surface
Purity:
Protein A/G affinity
Uniprot #:
P09326