NSJ Bioreagents

Recombinant CD86 Antibody

Product Code:
 
NSJ-V9474
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
C86/6500R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant CD86 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC staining of FFPE human lymph node tissue with recombinant CD86 antibody (clone C86/6500R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human lymph node tissue with recombinant CD86 antibody (clone C86/6500R) at 2ug/ml in PBS for 30min RT. Staining of cell surface is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free CD86 antibody (clone C86/6500R) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human lymph node tissue with recombinant CD86 antibody (clone C86/6500R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node tissue with recombinant CD86 antibody (clone C86/6500R) at 2ug/ml in PBS for 30min RT. Staining of cell surface is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD86 antibody (clone C86/6500R) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9474-20UG20 ug£264.00
Quantity:
NSJ-V9474-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 66-195 was used as the immunogen for the recombinant CD86 antibody.
Localisation:
Cell Surface
Purity:
Protein A/G affinity
Uniprot #:
P4208