NSJ Bioreagents

Recombinant CDH16 Antibody / Cadherin 16

Product Code:
 
NSJ-V9477SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CDH16/7028R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant CDH16 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC staining of FFPE human thyroid tissue with recombinant CDH16 antibody (clone CHD16/7027R) at 2ug/ml in PBS for 30min RT. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human renal cell carcinoma tissue with recombinant CDH16 antibody (clone CHD16/7027R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant CDH16 antibody (clone CDH16/7028R) as confirmation of integrity and purity.
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IHC staining of FFPE human thyroid tissue with recombinant CDH16 antibody (clone CHD16/7027R) at 2ug/ml in PBS for 30min RT. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cell carcinoma tissue with recombinant CDH16 antibody (clone CHD16/7027R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CDH16 antibody (clone CDH16/7028R) as confirmation of integrity and purity.

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NSJ-V9477SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin/cadherin 16/CDH16. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human full-length protein was used as the immunogen for the recombinant CDH16 antibody.
Localisation:
Cell Surface and Cytoplasmic
Purity:
Protein A affinity
Uniprot #:
O75309