NSJ Bioreagents

p27Kip1 Antibody / p27 / CDKN1B

Product Code:
 
NSJ-V9478
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
KIP1/1357
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the p27Kip1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC staining of FFPE human colon tissue with p27Kip1 antibody (clone KIP1/1357) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human prostate tissue using (clone KIP1/1357) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE human brain tissue with p27Kip1 antibody (clone KIP1/1357) at 2ug/ml in PBS for 30min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free p27Kip1 antibody (clone KIP1/1357) as confirmation of integrity and purity.
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IHC staining of FFPE human colon tissue with p27Kip1 antibody (clone KIP1/1357) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate tissue using (clone KIP1/1357) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human brain tissue with p27Kip1 antibody (clone KIP1/1357) at 2ug/ml in PBS for 30min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free p27Kip1 antibody (clone KIP1/1357) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9478-20UG20 ug£264.00
Quantity:
NSJ-V9478-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human CDKN1B protein was used as the immunogen for the p27Kip1 antibody.
Localisation:
Nuclear
Purity:
Protein A/G affinity
Uniprot #:
P46527