NSJ Bioreagents

ANXA1 Antibody / Annexin A1

Product Code:
 
NSJ-V9509SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
rANXA1/6451
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the ANXA1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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IHC staining of FFPE human tonsil tissue with ANXA1 antibody (clone rANXA1/6451). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human tonsil tissue with ANXA1 antibody (clone rANXA1/6451). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human A549 cell lysate using ANXA1 antibody (clone rANXA1/6451). Predicted molecular weight ~36 kDa.
4 / 4
SDS-PAGE analysis of purified, BSA-free ANXA1 antibody (clone rANXA1/6451) as confirmation of integrity and purity.
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IHC staining of FFPE human tonsil tissue with ANXA1 antibody (clone rANXA1/6451). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with ANXA1 antibody (clone rANXA1/6451). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human A549 cell lysate using ANXA1 antibody (clone rANXA1/6451). Predicted molecular weight ~36 kDa.
SDS-PAGE analysis of purified, BSA-free ANXA1 antibody (clone rANXA1/6451) as confirmation of integrity and purity.

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NSJ-V9509SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human Annexin A1 protein was used as the immunogen for the ANXA1 antibody.
Localisation:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P04083