NSJ Bioreagents

Recombinant CD20 Antibody

Product Code:
 
NSJ-V9519SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MS4A1/7015R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the recombinant CD20 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 6
IHC staining of FFPE human tonsil tissue with recombinant CD20 antibody (clone MS4A1/7015R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human tonsil tissue with recombinant CD20 antibody (clone MS4A1/7015R). Negative control inset: PBS instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 6
Negative control: IHC staining of FFPE human brain tissue with recombinant CD20 antibody (clone MS4A1/7015R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 6
Negative control: IHC staining of FFPE human heart tissue with recombinant CD20 antibody (clone MS4A1/7015R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of human Raji cell lysate using recombinant CD20 antibody (clone MS4A1/7015R). Predicted molecular weight ~33 kDa.
6 / 6
SDS-PAGE analysis of purified, BSA-free recombinant CD20 antibody (clone MS4A1/7015R) as confirmation of integrity and purity.
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IHC staining of FFPE human tonsil tissue with recombinant CD20 antibody (clone MS4A1/7015R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant CD20 antibody (clone MS4A1/7015R). Negative control inset: PBS instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant CD20 antibody (clone MS4A1/7015R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human heart tissue with recombinant CD20 antibody (clone MS4A1/7015R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Raji cell lysate using recombinant CD20 antibody (clone MS4A1/7015R). Predicted molecular weight ~33 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant CD20 antibody (clone MS4A1/7015R) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9519SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 97-297 was used as the immunogen for the recombinant CD20 antibody.
Localisation:
Cell surface
Purity:
Protein A affinity
Uniprot #:
P11836