NSJ Bioreagents

Recombinant CD86 Antibody

Product Code:
 
NSJ-V9521SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
rC86/6872
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant CD86 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 2
IHC staining of FFPE human lymph node with recombinant CD86 antibody (clone rC86/6872) at 2ug/ml in PBS for 30min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 2
SDS-PAGE analysis of purified, BSA-free recombinant CD86 antibody (clone rC86/6872) as confirmation of integrity and purity.
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IHC staining of FFPE human lymph node with recombinant CD86 antibody (clone rC86/6872) at 2ug/ml in PBS for 30min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD86 antibody (clone rC86/6872) as confirmation of integrity and purity.

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NSJ-V9521SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkins disease.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 66-195 was used as the immunogen for the recombinant CD86 antibody.
Localisation:
Cell Surface
Purity:
Protein A/G affinity
Uniprot #:
P4208