NSJ Bioreagents

CTSD Antibody / Cathepsin D

Product Code:
 
NSJ-V9530
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CTSD/4497
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the CTSD antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 6
IHC staining of FFPE human liver tissue with CTSD antibody (clone CTSD/4497). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human liver carcinoma in colon tissue with CTSDantibody (clone CTSD/4497). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human pancreas tissue with Cathepsin D antibody (clone CTSD/4497). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 6
SDS-PAGE analysis of purified, BSA-free CTSD antibody (clone CTSD/4497) as confirmation of integrity and purity.
5 / 6
Western blot testing of human liver tissue lysate using CTSD antibody (clone CTSD/4497).
6 / 6
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CTSD antibody (clone CTSD/4497). These results demonstrate the foremost specificity of the CTSD/4497 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

IHC staining of FFPE human liver tissue with CTSD antibody (clone CTSD/4497). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver carcinoma in colon tissue with CTSDantibody (clone CTSD/4497). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreas tissue with Cathepsin D antibody (clone CTSD/4497). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CTSD antibody (clone CTSD/4497) as confirmation of integrity and purity.
Western blot testing of human liver tissue lysate using CTSD antibody (clone CTSD/4497).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CTSD antibody (clone CTSD/4497). These results demonstrate the foremost specificity of the CTSD/4497 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V9530-20UG20 ug£264.00
Quantity:
NSJ-V9530-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins. It is synthesized as an inactive 43kDa preprocathepsin D that is cleaved and glycosylated to form a 46kDa procathepsin D and then further cleaved to produce 28kDa and 15kDa subunits (heavy and light chains, respectively). Cathepsin D exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimers disease.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Purified His-tagged CTSD protein was used as the immunogen for the CTSD antibody.
Localisation:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P07339