NSJ Bioreagents

Calbindin 2 Antibody / CALB2 / Calretinin

Product Code:
 
NSJ-V9543
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CALB2/7029R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Calbindin 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human testis tissue lysate using Calbindin 2 antibody (clone CALB2/7029R). Predicted molecular weight ~29 kDa.
2 / 4
IHC staining of FFPE human brain tissue with Calbindin 2 antibody (clone CALB2/7029R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
SDS-PAGE analysis of purified, BSA-free Calbindin 2 antibody (clone CALB2/7029R) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Calbindin 2 antibody (clone CALB2/7029R). These results demonstrate the foremost specificity of the CALB2/7029R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Western blot testing of human testis tissue lysate using Calbindin 2 antibody (clone CALB2/7029R). Predicted molecular weight ~29 kDa.
IHC staining of FFPE human brain tissue with Calbindin 2 antibody (clone CALB2/7029R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Calbindin 2 antibody (clone CALB2/7029R) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Calbindin 2 antibody (clone CALB2/7029R). These results demonstrate the foremost specificity of the CALB2/7029R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9543-20UG20 ug£264.00
Quantity:
NSJ-V9543-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
It recognizes a protein of about 29kDa, which is identified as Calretinin (also known as Calbindin 2). Calretinin is a vitamin D-dependent calcium-binding protein involved in calcium signaling. It is present in subsets of neurons throughout the brain and spinal chord, including sensory ganglia. Antibody to calretinin is useful in differentiating mesothelioma from adenocarcinomas of the lung. It also aids in differentiating adrenal cortical neoplasms from pheochromocytomas.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 23-242 was used as the immunogen for the Calbindin 2 antibody.
Localisation:
Cytoplasm, Nucleus
Purity:
Protein A affinity
Uniprot #:
P22676