NSJ Bioreagents

Recombinant Cystic Fibrosis Transmembrane Regulator Antibody / CFTR

Product Code:
 
NSJ-V9549SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CFTR/7154R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant Cystic Fibrosis Transmembrane Regulator antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC staining of FFPE human salivary gland with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human pancreas tissue with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE human lung carcinoma tissue with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
IHC staining of FFPE human spleen tissue with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human salivary gland with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreas tissue with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung carcinoma tissue with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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NSJ-V9549SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 258-385 was used as the immunogen for the recombinant Cystic Fibrosis Transmembrane Regulator antibody.
Localisation:
Cell surface, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P13569