NSJ Bioreagents

FOXL1 Antibody

Product Code:
 
NSJ-V9554
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-FOXL1-1F8
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the FOXL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Immunofluorescent staining of PFA-fixed human HeLa cells using FOXL1 antibody (green, clone PCRP-FOXL1-1F8) and phalloidin (red).
2 / 5
IHC staining of FFPE human prostate tissue with FOXL1 antibody (clone PCRP-FOXL1-1F8) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
FACS staining of PFA-fixed human HeLa cells with FOXL1 antibody (blue, clone PCRP-FOXL1-1F8), and unstained cells (red).
4 / 5
SDS-PAGE analysis of purified, BSA-free FOXL1 antibody (clone PCRP-FOXL1-1F8) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using FOXL1 antibody (clone PCRP-FOXL1-1F8). These results demonstrate the foremost specificity of the PCRP-FOXL1-1F8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using FOXL1 antibody (green, clone PCRP-FOXL1-1F8) and phalloidin (red).
IHC staining of FFPE human prostate tissue with FOXL1 antibody (clone PCRP-FOXL1-1F8) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS staining of PFA-fixed human HeLa cells with FOXL1 antibody (blue, clone PCRP-FOXL1-1F8), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free FOXL1 antibody (clone PCRP-FOXL1-1F8) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using FOXL1 antibody (clone PCRP-FOXL1-1F8). These results demonstrate the foremost specificity of the PCRP-FOXL1-1F8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9554-20UG20 ug£264.00
Quantity:
NSJ-V9554-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
FOXL1 is a 337 amino acid protein encoded by the mouse gene FOXL1. FOXL1 belongs to the forkhead family and contains one forkhead DNA-binding domain. The HNF3/forkhead family includes a large number of transcription factors that share a structurally related DNA binding domain. forkhead factors are known to play important roles both during development and in adults. FOXL1 is a winged helix transcriptional regulator expressed in the mesenchymal layer of developing and mature gastrointestinal tract. FOXL1-deficient mice exhibit various defects not only in the epithelial layer of the gastrointestinal tract but also in gut-associated lymphoid tissues. In the small intestine of FOXL1-deficient mice, the formation of Peyers patches is affected, particularly in the caudal region. FOXL1 is a mesenchymal modifier of the Adenomatous Polyposis Coli (APC) gene products and plays a key role in gastrointestinal tumorigenesis.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 104-258 was used as the immunogen for the FOXL1 antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q12951