NSJ Bioreagents

Superoxide Dismutase 1 Antibody / SOD1

Product Code:
 
NSJ-V9586
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SOD1/3923
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the Superoxide Dismutase 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Immunofluorescent staining of PFA-fixed human MCF-7 cells Superoxide Dismutase 1 antibody (green, clone SOD1/3923) and phalloidin (red).
2 / 5
IHC staining of FFPE human prostate tissue with Superoxide Dismutase 1 antibody (clone SOD1/3923) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
FACS staining of PFA-fixed human MCF-7 cells with Superoxide Dismutase 1 antibody (blue, clone SOD1/3923), and unstained cells (red).
4 / 5
SDS-PAGE analysis of purified, BSA-free Superoxide Dismutase 1 antibody (clone SOD1/3923) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Superoxide Dismutase 1 antibody (clone SOD1/3923). These results demonstrate the foremost specificity of the SOD1/3923 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human MCF-7 cells Superoxide Dismutase 1 antibody (green, clone SOD1/3923) and phalloidin (red).
IHC staining of FFPE human prostate tissue with Superoxide Dismutase 1 antibody (clone SOD1/3923) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS staining of PFA-fixed human MCF-7 cells with Superoxide Dismutase 1 antibody (blue, clone SOD1/3923), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free Superoxide Dismutase 1 antibody (clone SOD1/3923) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Superoxide Dismutase 1 antibody (clone SOD1/3923). These results demonstrate the foremost specificity of the SOD1/3923 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9586-20UG20 ug£264.00
Quantity:
NSJ-V9586-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Cu-Zn superoxide dismutase-1 (SOD-1) is a well-characterized cytosolic scavenger of oxygen free radicals that requires copper and zinc binding to potentiate its enzymatic activity. Enzymatically, SOD-1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. SOD-1 is ubiquitously expressed in somatic cells and functions as a homodimer. Defects in the gene encoding SOD-1 have been implicated in the progression of neurological diseases, including amyotrophic lateral sclerosis (ALS), a neurodegenerative disease characterized by the loss of spinal motor neurons, Down syndrome and Alzheimer s disease. In familial ALS, several mutations in SOD-1 predominate, resulting in the loss of zinc binding, the loss of scavenging activity of SOD-1, and correlate with an increase in neurotoxicity and motor neuron death.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the Superoxide Dismutase 1 antibody.
Localisation:
Cytoplasmic, nuclear
Purity:
Protein A/G affinity
Uniprot #:
P00441