NSJ Bioreagents

TDRKH Antibody

Product Code:
 
NSJ-V9602
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-TDRKH-1H2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the TDRKH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Immunofluorescent staining of PFA-fixed human HeLa cells using TDRKH antibody (green, clone PCRP-TDRKH-1H2) and phalloidin (red).
2 / 5
IHC staining of FFPE human ovarian carcinoma tissue with TDRKH antibody (clone PCRP-TDRKH-1H2) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
FACS staining of PFA-fixed human HeLa cells with TDRKH antibody (blue, clone PCRP-TDRKH-1H2), and unstained cells (red).
4 / 5
SDS-PAGE analysis of purified, BSA-free TDRKH antibody (clone PCRP-TDRKH-1H2) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TDRKH antibody (clone PCRP-TDRKH-1H2). These results demonstrate the foremost specificity of the PCRP-TDRKH-1H2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using TDRKH antibody (green, clone PCRP-TDRKH-1H2) and phalloidin (red).
IHC staining of FFPE human ovarian carcinoma tissue with TDRKH antibody (clone PCRP-TDRKH-1H2) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS staining of PFA-fixed human HeLa cells with TDRKH antibody (blue, clone PCRP-TDRKH-1H2), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free TDRKH antibody (clone PCRP-TDRKH-1H2) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TDRKH antibody (clone PCRP-TDRKH-1H2). These results demonstrate the foremost specificity of the PCRP-TDRKH-1H2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9602-20UG20 ug£264.00
Quantity:
NSJ-V9602-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human TDRKH protein was used as the immunogen for the TDRKH antibody.
Localisation:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q9Y2W6