NSJ Bioreagents

KLF12 Antibody

Product Code:
 
NSJ-V9604
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-KLF12-1E3
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Aliquot the KLF12 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using KLF12 antibody (green, clone PCRP-KLF12-1E3) and Phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with KLF12 antibody (blue, clone PCRP-KLF12-1E3), and unstained cells (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free KLF12 antibody (clone PCRP-KLF12-1E3) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using KLF12 antibody (clone PCRP-KLF12-1E3). These results demonstrate the foremost specificity of the PCRP-KLF12-1E3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed human HeLa cells using KLF12 antibody (green, clone PCRP-KLF12-1E3) and Phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with KLF12 antibody (blue, clone PCRP-KLF12-1E3), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free KLF12 antibody (clone PCRP-KLF12-1E3) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using KLF12 antibody (clone PCRP-KLF12-1E3). These results demonstrate the foremost specificity of the PCRP-KLF12-1E3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9604-20UG20 ug£264.00
Quantity:
NSJ-V9604-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Description:
Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. KLF12 is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Repression by the encoded protein requires binding with a corepressor, CtBP1. Two transcript variants encoding different isoforms have been found for this gene.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the KLF12 antibody.
Localisation:
Nucleus, cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q9Y4X4