NSJ Bioreagents

ECD Antibody / SGT1

Product Code:
 
NSJ-V9605
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-ECD-1D10
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Aliquot the ECD antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using ECD antibody (green, clone PCRP-ECD-1D10) and Phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with ECD antibody (blue, clone PCRP-ECD-1D10), and unstained cells (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free ECD antibody (clone PCRP-ECD-1D10) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ECD antibody (clone PCRP-ECD-1D10). These results demonstrate the foremost specificity of the PCRP-ECD-1D10 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using ECD antibody (green, clone PCRP-ECD-1D10) and Phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with ECD antibody (blue, clone PCRP-ECD-1D10), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free ECD antibody (clone PCRP-ECD-1D10) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ECD antibody (clone PCRP-ECD-1D10). These results demonstrate the foremost specificity of the PCRP-ECD-1D10 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V9605-20UG20 ug£264.00
Quantity:
NSJ-V9605-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Description:
Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the ECD antibody.
Localisation:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
O95905