NSJ Bioreagents

JAZF1 Antibody

Product Code:
 
NSJ-V9613
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-JAZF1-1C2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the JAZF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Immunofluorescent staining of PFA-fixed human HeLa cells using JAZF1 antibody (green, clone PCRP-JAZF1-1C2) and phalloidin (red).
2 / 5
IHC staining of FFPE human testis tissue with JAZF1 antibody (clone PCRP-JAZF1-1C2) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
FACS staining of PFA-fixed human HeLa cells with JAZF1 antibody (blue, clone PCRP-JAZF1-1C2), and unstained cells (red).
4 / 5
SDS-PAGE analysis of purified, BSA-free JAZF1 antibody (clone PCRP-JAZF1-1C2) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using JAZF1 antibody (clone PCRP-JAZF1-1C2). These results demonstrate the foremost specificity of the PCRP-JAZF1-1C2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using JAZF1 antibody (green, clone PCRP-JAZF1-1C2) and phalloidin (red).
IHC staining of FFPE human testis tissue with JAZF1 antibody (clone PCRP-JAZF1-1C2) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS staining of PFA-fixed human HeLa cells with JAZF1 antibody (blue, clone PCRP-JAZF1-1C2), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free JAZF1 antibody (clone PCRP-JAZF1-1C2) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using JAZF1 antibody (clone PCRP-JAZF1-1C2). These results demonstrate the foremost specificity of the PCRP-JAZF1-1C2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9613-20UG20 ug£264.00
Quantity:
NSJ-V9613-100UG100 ug£534.00
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This product comes from: United States.
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
JAZF1 (juxtaposed with another zinc finger protein 1), also known as TIP27 (TAK1-interacting protein 27) or ZNF802 (zinc finger protein 802), is a 243 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers. Existing as multiple alternatively spliced isoforms, JAZF1 interacts with the nuclear orphan receptor TR4 and is thought to function as a transcriptional repressor, effectively down-regulation the expression of TR4. Chromosomal aberrations in the gene encoding JAZF1 are associated with the pathogenesis of endometrial stromal tumors, suggesting a role for JAZF1 in carcinogenesis.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 1-130 was used as the immunogen for the JAZF1 antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q86VZ6