NSJ Bioreagents

ZNF358 Antibody

Product Code:
 
NSJ-V9614
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2c
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-ZNF358-1A6
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the ZNF358 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells ZNF358 antibody (green, clone PCRP-ZNF358-1A6) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with ZNF358 antibody (blue, clone PCRP-ZNF358-1A6), and unstained cells (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free ZNF358 antibody (clone PCRP-ZNF358-1A6) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZNF358 antibody (clone PCRP-ZNF358-1A6). These results demonstrate the foremost specificity of the PCRP-ZNF358-1A6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells ZNF358 antibody (green, clone PCRP-ZNF358-1A6) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with ZNF358 antibody (blue, clone PCRP-ZNF358-1A6), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free ZNF358 antibody (clone PCRP-ZNF358-1A6) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZNF358 antibody (clone PCRP-ZNF358-1A6). These results demonstrate the foremost specificity of the PCRP-ZNF358-1A6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9614-20UG20 ug£264.00
Quantity:
NSJ-V9614-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
Description:
Zinc finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc finger proteins contain a Kr ppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. As a member of the Kr ppel C2H2-type zinc finger protein family, ZNF358 (zinc finger protein 358) is a 481 amino acid nuclear protein that contains nine C2H2-type zinc fingers through which it is thought to be involved in DNA-binding and transcriptional regulation.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the ZNF358 antibody.
Localisation:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q9NW07