NSJ Bioreagents

Recombinant VIM Antibody / Vimentin

Product Code:
 
NSJ-V9616
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
rVIM/6914
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the recombinant VIM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot testing of human U-87 MG cell lysate using recombinant VIM antibody (clone rVIM/6914). Predicted molecular weight ~53 kDa.
2 / 3
IHC staining of FFPE human tonsil tissue with recombinant VIM antibody (clone rVIM/6914). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant VIM antibody (clone rVIM/6914) as confirmation of integrity and purity.
❮❯

Western blot testing of human U-87 MG cell lysate using recombinant VIM antibody (clone rVIM/6914). Predicted molecular weight ~53 kDa.
IHC staining of FFPE human tonsil tissue with recombinant VIM antibody (clone rVIM/6914). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant VIM antibody (clone rVIM/6914) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9616-20UG20 ug£264.00
Quantity:
NSJ-V9616-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
Description:
When used in conjunction with anti-keratin staining, anti-vimentin staining is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 366-466 was used as the immunogen for the recombinant VIM antibody.
Localisation:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P08670